Contents
page of the issue April 2008
EQUINE
L. Szeredi, Zs. Kléh, V.
Pálfi: Equine viral arteritis virus caused disease in a
foal in a Hungarian horse farm. Case report / 195
PORCINE
P. Papócsi, A. Hermán, J.
Gundel, S. Gy. Fekete: Effect of chopped alfalfa hay
supplementation of the feedstuffs during pregnancy on breeding sows’
reproductive performance / 199
SMALL
ANIMALS
Á. Maróti-Agóts, A.
Markó, L. Zöldág: Modified molecular genetic
diagnosis of feline polycystic kidney disease / 205
A. Tipold: Inflammatory diseases of
small animals’ central nervous system / 212
WILD
ANIMALS
V. Molnár, N. Váradi, A.
Beregi, B. Fenyves, E. Sós, M. Liptovszky, B. Bakos, Z.
Molnár, F. Felkai: Radiology of bats (Chiroptera) / 219
REPRODUCTIVE
BIOLOGY
G. Horváth, L. Solti: Use
of chemically defined media for the vitrification of bovine oocytes /
227
LABORATORY
DIAGNOSTICS
L. Szeredi, Á. Ráczné
Mészáros: The use of immunohistochemistry for the
diagnosis of infectious animal diseases / 232
INSTRUMENTAL
DIAGNOSTICS
L. Tóth, Zs. Petrási, P.
Bogner: Possible applications of magnetic resonance imaging
(MRI) in the animal sciences / 247
EVENTS
Veterinary Memorial Day in
Fejér County (Székesfehérvár, 7 December
2007) (A. Szabó) / 255
IN
MEMORIAM
János Szabó
Szűcs (1926–2007) (K. Almási) / 211
L. Szeredi – Zs.
Kléh – V. Pálfi:
EQUINE
VIRAL ARTERITIS VIRUS CAUSED DISEASE IN A FOAL IN A HUNGARIAN HORSE
FARM. CASE REPORT
Abortion and respiratory
diseases with fever was observed in newborn foals in a small farm. One
of the newborn foals died some hours after the onset of symptoms, and
the lungs were sent for pathological and microbiological examinations.
Severe interstitial pneumonia with the presence of hyaline membrane and
mild lympho-histiocytic vasculitis, perivasculitis and fibrinoid
necrosis in the small muscular arteries was observed. Equine arteritis
virus (EAV) was isolated from the lung and large amount of virus
antigen was detected in the lung with immunohistochemistry using a
commercially available EAV-specific monoclonal antibody. This case
indicates, that EAV-induced disease can rarely occur even nowadays in
Hungary, in spite of the high seroprevalence of EAV infection in the
country.
P. Papócsi – A.
Hermán – J. Gundel – S. Gy. Fekete:
EFFECT
OF CHOPPED ALFALFA HAY SUPPLEMENTATION OF THE FEEDSTUFFS DURING
PREGNANCY ON BREEDING SOWS’ REPRODUCTIVE PERFORMANCE
The authors compared the
effect of an average diet (control) and a basic diet, the control was
supplemented with 300 g chopped alfalfa hay daily, to evaluate the
optimum fibre supply of the pregnant sows during two whole reproductive
cycles. According to their results the lactational weight loss
decreased and the voluntary feed intake increased after the application
of fibre supplement. Alfalfa supplementation increased the litter size
at parturition and the number and daily gain of suckling piglets. These
results confirm the beneficial effect of the fibre supplementation,
especially in form of the used special alfalfa preparation.
Á.
Maróti-Agóts – A. Markó – L. Zöldág:
MODIFIED
MOLECULAR GENETIC DIAGNOSIS OF FELINE POLYCYSTIC KIDNEY DISEASE
Polycystic kidney disease
(PKD) is one of the most common inherited feline diseases of nowadays,
affecting 38% of Persian and Persian-related cats worldwide, including
the British Shorthair, Maine Coon, Exotic Shorthair, Himalaya and in
Siamese. The way of inheritance of PKD is proven to be autosomal
dominant. During the pathogenesis of the disease, fluid-filled cysts
evolve in the kidney and sometimes in other organs, such as the liver,
uterus or the pancreas. The cysts cause the atrophy of the kidney’s
matter followed by chronic renal failure. The aim of this study was to
determine the value of molecular diagnosis of feline PKD, from both new
and fixed histological samples. Total genomic DNA was extracted and
purified, the mutated sequences (PKD1 gene, exon29) were amplified,
sequenced and digested by restriction endonuclease (RFLP), finally
separated by electrophoresis. The developed allele specific PCR
reaction was successfully applied for diagnosis in a special three
primer containing PCR reaction (Figure). Molecular examination of PKD
is well-suited for practical application and can be used for routine
early diagnosis, screening, and reducing the incidence of warranty
cases.
V. Molnár – N.
Váradi – A. Beregi – B. Fenyves – E. Sós – M. Liptovszky
– B. Bakos – Z. Molnár – F. Felkai:
RADIOLOGY
OF BATS (CHIROPTERA)
The most common
radiological indications in bats are traumatic injuries of the sceletal
system. With the exception of the lungs the radioopacity of the soft
tissue organs are quite similar, so there is no justification in bats
for the so called hard and soft X-ray techniques used in larger animal
species.
Radiology contributes a lot
of valuable information to the diagnostic assessment of injured bats.
It is indispensable to the correct charting of the alterations, the
choice of the proper therapy, the planning and the control of the
surgical intervention and the efficacy of regeneration processes.
Neither the form and the
size, nor the function of the soft tissue organs can be diagnosed by
the evaluation of the radiographs, which raises the need of other
diagnostic methods during the basic clinical steps.
G. Horváth – L.
Solti:
USE
OF CHEMICALLY DEFINED MEDIA FOR THE VITRIFICATION OF BOVINE OOCYTES
Most media used during
oocyte vitrification contains protein sources of animal origin, which
may raise epidemiological concerns, and as these compounds are not
defined chemically, the outcome of vitrification may vary, as well. The
aim of the study was to exclude proteins of animal origin from media
and to replace it with chemically defined compounds. Prior to
vitrification, the oocytes were pretreated with cholesterol loaded into
methyl-β-cyclodextrin or fetuin. After vitrification, the cholesterol
treated group of oocytes resulted in higher cleavage rate and
8-cell-stage rate in comparison to the untreated oocytes or the ones
vitrified with the traditional fetal calf serum containing media, but
no effect was seen in their further development. The fetuin
supplementation before vitrification did not result in any advantage,
but the results were comparable to the standard process, during which
fetal calf serum was used.
L. Szeredi – Á.
Ráczné Mészáros:
THE
USE OF IMMUNOHISTOCHEMISTRY FOR THE DIAGNOSIS OF INFECTIOUS ANIMAL
DISEASES
After a brief review of the
immunohistochemical method (IHC), the authors present the IHC protocols
used in their laboratory to detect infectious diseases. They report
about 35 different, mostly commercially available primary antibodies,
which are able to detect viruses, bacteria, fungi or parasites in
formaldehyde-fixed and paraffin-embedded tissue samples. They review
the sensitivity and specificity of IHC in case of each infectious
disease according to the literature, and they present also their own
experiences. The authors suggest the use of IHC in the daily diagnostic
work, because of its rapidity, sensitivity and the relative low cost of
the test.
L. Tóth – Zs.
Petrási – P. Bogner:
POSSIBLE
APPLICATIONS OF MAGNETIC RESONANCE IMAGING (MRI) IN THE ANIMAL SCIENCE
The autors review the
possible applications of one of the modern imaging techniques, magnetic
resonance imaging (MRI). The authors summarize the fields of
application of MRI in animal science, particularly the possibility of
usage of MRI in veterinary therapy. Finally, they present the basis and
potentials of functional MRI, and review the advantages and limits of
the use of MR imaging.