Contents page of the issue May 2007




RUMINANTS
K. Ary, Á. Cs. Bajcsy, I. Kacskovics, I. Mádl, L. Takács, O. Szenci: Methods for the detection of immunoglobulin concentration of the colostrum and in newborn calf and domestic experiences on the use of Midland-fast test / 259
L. Szeredi, Sz. Jánosi, M. Tenk, L. Tekes, M. Bozsó, Z. Deim, T. Molnár: Epidemiological and pathological study on the causes of abortion in sheep and goats in Hungary (1998–2005). Short secondary communication / 269

ANIMAL WELFARE
Recommendation of the Scientific Animal Experiment Committee of the Hungarian Academy of Science on the teaching of participants in animal experiments (L. Bertók) / 276

SMALL ANIMALS
J. M. Steiner: Diagnosis of pancreatitis in dogs and cats / 277

PET ANIMALS
A. Beregi, N. Biró, V. Molnár, E. Sós: Ultrasonic examination in snakes and lizards / 282

PHARMACOLOGY
D. Szécsényi, P. Vajdovich, T. Gaál: Role of pump proteins causing therapy resistance in different diseases Literature review. Part 1 / 295

ANDROLOGY
A. Kovács, Kh. Tumennasan, Sh. Demberel, Sz. Nagy, G. Kútvölgyi, J. Oláh, A. Jávor: Cryopreservation of Argali spermatozoa. Preliminary communication / 306

LABORATORY DIAGNOSTICS
Cs. Jakab, J. Halász, A. Kiss, Zs. Schaff, M. Pekár, R. Keszthelyi, Á. Meczker, J. Kulka: Tissue microseries (tissue micro-array – TMA) technique in the veterinary oncopathological practice / 310

EVENTS
17th Congress of the Hungarian Buiatric Society (Gyula, 8–10th October, 2006) (L. Ózsvári) / 315

JUBILEE
János Mészáros is 80 years old (T. Kassai, K. Molnár) / 317

BOOK REVIEW
P. Markovits: Stets bei Verstand sein (Always being in right mind) (L. Visnyei) / 268
M. Hőnich: Picture on the frequent diseases of fair games (L. Visnyei) / 276

K. Ary – Á. Cs. Bajcsy – I. Kacskovics – I. Mádl – L. Takács – O. Szenci:
METHODS FOR THE DETECTION OF IMMUNOGLOBULIN CONCENTRATION OF THE COLOSTRUM AND IN NEWBORN CALF AND DOMESTIC EXPERIENCES ON THE USE OF MIDLAND-FAST TEST

The authors briefly present the methods used for the detection of immunoglobulin concentration of the colostrum and blood: colostrometer, refractometer, lateral immunodiffusion test, radial immunodiffusion test, ELISA test (colostrum) and natrium-sulphite precipitation probe, zink-sulphate probe, glutaraldehyde-coagulation probe, latexagglutination test, simple immunodiffusion test, electrophoresis (blood sample). They measured the immunoglobulin concentration of 33 colostrum and blood samples with Midland lateral immunodiffusion fast test and evaluated them from the point of view of sensitivity, specificity, positive and negative prognosis. The real immunoglobulin concentration was established by SRID (single radial immunodiffusion) test. The results are in Table 4 and 5. According to it, Midland-fast test gives the opportunity to follow up immunoglobulin-supply of calves in dairies.

A. Beregi – N. Bíró – V. Molnár – E. Sós:
ULTRASOUND EXAMINATION IN SNAKES AND LIZARDS

It is getting more and more common to keep reptiles as pets at homes and the number of rare and valuable species kept in private collections increased recently, as well.
By the ultrasonographic examination of reptiles, veterinary clinicians may obtain additional information that can be used in the diagnostic and therapeutic work. In this study 17 lizards (6 species), 3 caimans (1 species) and 15 snakes (7 species) were examined. The use of ultrasound in diagnostic procedures, the preparation of the patient, the methods and techniques and the echoanatomy of snakes and lizards are described. The study summarises the basic technical conditions of ultrasonography and point out the anatomical and physiological characteristics that could decrease the efficiency of ultrasonographic examinations.
According to this study, it is established that, in comparison with other diagnostic methods (palpation, haematological examination, laparoscopy and radiography), ultrasonography offers a non-invasive and reliable technique to diagnose diseases in snakes and lizards.

D. Szécsényi – P. Vajdovich – T. Gaál:
ROLE OF PUMP PROTEINS CAUSING THERAPY RESISTANCE IN DIFFERENT DISEASES. LITERATURE REVIEW. PART 1

In spite of seemingly proper drug selection, it is not uncommon that the drug therapy fails to result as expected (curing, remission). This phenomenon is called therapy-resistance in medical science. It was first recognized in connection with antibacterial drugs (antibiotic resistance), but later, during chemotherapeutic treatment of neoplastic diseases, the therapy-resistance of some tumour cells made scientists to focus their research on the causes and elimination of this resistance. In the first part of the study, the authors give an overview of the concept of therapy-resistance, and of the mechanism of bacterial antibiotic resistance. In connection to the therapy-resistance of tumour cells, they describe the function, structure and substrate specificity of „efflux-pumps”, especially „ABC-transporters”, because these molecules play the most important role in this type of treatment-failure.

A. Kovács – Kh. Tumennasan – Sh. Demberel – Sz. Nagy – G. Kútvölgyi – J. Oláh – A. Jávor:
CRYOPRESERVATION OF ARGALI SPERMATOZOA. PRELIMINARY COMMUNICATION

Collection and cryopreservation of epididymal spermatozoa of a shot Argali ram was carried out in Mongolia. According to the viability and acrosome staining finished and evaluated in Hungary, 21.5% of the frozen/thawed spermatozoa remained alive and intact, proving that the genetic material of shot individuals may be saved also in this species. Further experiments and using the frozen spermatozoa to artificial insemination of domestic sheep are planned.

Cs. Jakab – J. Halász – A. Kiss – Zs. Schaff – M. Pekár – R. Keszthelyi – Á. Meczker – J. Kulka:
TISSUE MICROSERIES (TISSUE MICRO-ARRAY – TMA) TECHNIQUE IN THE VETERINARY ONCOPATHOLOGICAL PRACTICE

In the present study the authors describe the Tissue Micro-Array (TMA) also known as the tissue microseries technique, a novel histopathological procedure currently applied in the pathological practice. During TMA tissue microseries capable for storing more than a thousand specimens are obtained by manually or automatically removing cylindrical tissue sections from classical paraffin-embedded blocks, following deparaffinization. The described method permits considerable savings of reagents and the examination of several specimens on only one slide. The authors also describe the laboratory manual instrument kit developed by Hungarian researchers, suitable for obtaining the tissue microseries, as well as the operations performed by using this kit: the marking of the representative tissue areas on the classical histopathological slides under light microscope, the removal of the areas of interest from the paraffin-embedded blocks, followed by the insertion of these tissue sections in tissue multiblocks presenting a series of holes of identical proportions and the preparation of histopathological sections. The authors also illustrate the procedures described in the present study.