Contents page of the issue February 2007




EQUINE

Z. Bakos, J. Tóth: Abdominal ultrasonography in the adult horse. Literature review and own experiences. Part 1. / 67

BOVINE
A. Pécsi, J. Földi, M. Kulcsár, T. Pécsi, Gy. Huszenicza: Complications of bacterial origin of the involution in cattle. Literature review. Part 2. / 77
L. Köcski, J. Mészáros: Effect of GnRH-treatment on the pregnancy of dairy cows / 84

PORCINE
L. Szeredi: Simultaneous detection of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV-2) in lungs of swine died of pneumonia / 90

POULTRY
L. Szeredi, N. Pálmai, K. Erdélyi, Z. Deim, L. Márton, R. Glávits: Detection of highly pathogenic avian influenza virus, H5N1 with immunohistochemical method in mute swan (Cygnus olor) / 98
É. Ivanics, V. Palya, R. Glávits, Cs. Nemes, T. Mató, Á. Dán, Eöné Nagy, P. Zarka, A. Surányi, F. Muncz, T. Bacsó, L. Kőrösi: Re-emergence of the chicken anaemia virus in Hungary / 103

SMALL ANIMALS
C. Favrot, S. Wilhelm: Viral skin diseases of dogs and cats / 111

WILD ANIMALS
B. Egri, B. Húsvéth: The first time about the warble fly infection of roe-deer and red deer in Szigetköz / 121

LETTER TO THE EDITOR
Large bowel worm infestation more attention in horse merits! Remarks to the article of K. A. von Plocki: Colic diseases in horse. Review (T. Kassai) / 76

BOOK REVIEW
F. C. Stades, W. Neumann, H. H. Boeve, B. Spiss, M. Wymann: Practical veterinary ophthalmology. 3rd revised and extended edition (J. Kováts) / 127

Z. Bakos – J. Tóth:
ABDOMINAL ULTRASONOGRAPHY IN THE ADULT HORSE. LITERATURE REVIEW AND OWN EXPERIENCES. PART 1

The study describes the principles and methods of equine two-dimensional abdominal ultrasonography. Beside the review of the literature, the authors also present their own experiences. They demonstrate the imaging technique, physiological findings and the most important pathological alterations using figures and ultrasonograms. In the firs part they discuss the ultrasonography of the gastrointestinal tract.

A. Pécsi – J. Földi – M. Kulcsár – T. Pécsi – Gy. Huszenicza:
COMPLICATIONS OF BACTERIAL ORIGIN OF THE INVOLUTION IN CATTLE. LITERATURE REVIEW

In the 2nd part the authors summarise the main principles of the therapy used nowadays.

L. Köcski – J. Mészáros:
EFFECT OF GNRH-TREATMENT ON THE PREGNANCY OF DAIRY COWS

The authors treated, in two series of experiments, dairy cows with 50 μg of GnRH-analogue preparation (1.0 ml Gonavet Veyx) for the purpose of increasing progesterone production of corpus luteum (CL) and thus, increasing the chance of pregnancy. In the first experiment GnRH was given im. on 12th day after insemination following induced oestrus to cows with functioning CL (n=154). Compared to control cows (n=168), pregnancy rate was higher with 9.4% (p<0.01) in treated cows. Increase in pregnancy rate was significant in first calving cows (17.5%, p<0.01) and in case of first insemination after calving (14.4%, p<0.01). The difference was significant between second inseminations and inseminations within 200 days following calving.
In the second series of experiments 490 dairy cows of four dairies were treated im. with GnRH-analogue preparation on the 4th (5th) day following insemination for helping marked progesterone increase and faster embryo development. Compared to 492 control cows, in three dairies minimal difference was found in favour of treated animals.
The authors recommend in long-term the treatment around 12th day, easily built in reproductive technology, in dairies where oestrus and/or ovulation synchronisation is made regularly (by prosynch, ovsynch, etc. methods).

L. Szeredi:
SIMULTANEOUS DETECTION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS (PRRSV) AND PORCINE CIRCOVIRUS TYPE 2 (PCV-2) IN LUNGS OF SWINE DIED OF PNEUMONIA

Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV-2) were detected simultaneously with immunohistological (IH) examination in lungs of 5 growing pigs died of pneumonia, came from a large farm. Histological lesions characteristic for PCV-2 infection (occurrence of multinucleated giant cells) was observed only in 2 cases. Histological lesions correspond to secondary bacterial infection was always evident together with viral interstitial pneumonia. Pathogenic bacteria were isolated in three cases from the lungs (Pasteurella multocida two cases, Streptococcus spp. in one case). In two cases PCV-2 could be not observed in all five tissues samples of cranial part of the lungs, which were investigated in each animal, and in most of the cases the amount of virus-antigen showed great variance in the different tissue samples of the same animal. PRRSV was demonstrated in each tissue samples, and mostly in large amount with IH. PCV-2 and PRRSV were evident consistently in different cells, and simultaneous occurrence of the two viruses in the same cell was never observed using the double IH method. This is the first report about the detection of PRRSV with IH in Hungary, and it is also the first time of the demonstration of simultaneous occurrence of PRRSV and PCV-2 in lungs of swine died due to pneumonia in this country.

L. Szeredi – N. Pálmai – K. Erdélyi – Z. Deim – L. Márton – R. Glávits:
DETECTION OF HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUS, H5N1 WITH IMMUNOHISTOCHEMICAL METHOD IN MUTE SWAN (CYGNUS OLOR)

The presence of the highly pathogenic H5N1 avian influenza virus was examined with an immunohistochemical (IH) method in different organs of a mute swan (Cygnos olor) that died of the infection. The virus was detected in formaline-fixed and paraffin-embedded tissue samples of the brain, pancreas, liver, spleen, lung and small intestine, and mostly in the nucleus of different cell types by murine monoclonal antibodies specific for the nucleoprotein of type A influenza virus. The virus could be found in large amounts primarily in the brain. To the authors knowledge, avian influenza virus was demonstrated for the first time in the mute swan by immunohistochemistry.

É. Ivanics – V. Palya – R. Glávits – Cs. Nemes – T. Mató – Á. Dán – Eörné Nagy – P. Zarka – A. Surányi – F. Muncz – T. Bacsó – L. Kőrösi:
RE-EMERGENCE OF THE CHICKEN ANAEMIA VIRUS IN HUNGARY

After the outbreaks in 1970’s and 80’s the authors report the first re-emergence of chicken anaemia virus infection in several flocks during December 2005 and the beginning of 2006.
Deaths occurred between the age of 10–20 days, with a peak at the age of 14–17 days. Daily mortality rates were 1–1.5% with a 6–9% cumulative mortality during outbreaks.
Macroscopic and histological lesions comprised anaemia, stunted growth, yellowish discolouration of red bone marrow (with decreased numbers of erythroid, myeloid and thrombocyte precursor cells) and the general atrophy and lymphoid depletion of lymphatic organs (bursa of Fabricius, thymus and spleen). Inflammatory skin lesions on the head, wings and legs, caused by secondary bacterial infections (primarily by Clostridia and Staphylococci) were detected in all cases. Bacterial and fungal infections were also frequent in joints and other internal organs. Secondary infections were the major cause of the losses.
Diagnosis of the disease was based on the complex evaluation of clinical signs, macroscopic and microscopic lesions, demonstration of the presence of the causative circovirus and classification of secondary infections.
Serological tests confirmed that the affected day old chicks originated from initially sero-negative flock, which acquired the infection during the egg laying period.

B. Egri – B. Húsvéth:
THE FIRST TIME ABOUT THE WARBLE FLY INFECTION OF ROE-DEER AND RED DEER IN SZIGETKÖZ INFESTATION

The authors review the biology of the warble flies (Hypoderma diana and H. actaeon), analyse the warble fly-larvae infestations of 32 roe-deer and 51 red deer shot in huntings in Szigetköz Region of North-West Hungary in the summer, fall and winter of 2005–2006. Hypoderma larvae were found to occur in 74.5% of red deer and 84.37% of roe deer. As a result of identification of a total of 2601 larvae, the incidence of 2 species was established. The 1811 larvae of H. diana were found to occur in 84.3% of roe deer and 46.08% of red deer, while 790 larvae of H. actaeon showed 53.92% incidence in red deer.