Contents page of the issue Sept 2006



EQUINE

P. Tóth: Examination methods of the equine thoracolumbar and sacral region. Literature review / 515

PORCINE
Gy. Balka, M. Rusvai, S. Kecskeméti, I. Kiss: PRRS – new challenge in the porcine sector. 1. Characteristics of the virus. Literature review and own experiences / 524
V. Halas, M. Kovács, L. Babinszky: Impact of nutrient supply on the immune functions in pig. Literature review / 533

SMALL ANIMALS
P. Zenke, Zs. Pádár, L. Zöldág: Molecular genetics and dog breeding / 544
G. Liebisch, W. Thiet, A. Liebisch: Canine monocytic and granulocytic ehrlichiosis, two tick-born diseases of dogs in Germany / 551
R. Hämmerling: Alopecia of dogs: building up diagnosis from step to step / 558

FISH
K. Lefler, J. Gál, Gy. Demeter: Effect of water temperature on the embryonic development and hatching results of rainbow trout (Oncorhynchus mykiss) / 565

ACADEMIC REPORTS, 2006
Veterinary research in 2005 in the light of academic reports. Part 4 / 570

P. Tóth:
EXAMINATION METHODS OF THE EQUINE THORACOLUMBAR AND SACRAL REGION. LITERATURE REVIEW

The author summerizes the clinical examination methods of the equine vertebral region. The physical examination involves: inspection, palpation, flexion and manipulation tests, rectal examination, examination in movement and local anesthesia. The x-ray can be used in dorsoventral 30o oblique and ventrodorsal 20o oblique position in recumbent horse. The ultrasonographic examination can be performed transcutanly and per rectum. Scintigraphy and infrared termography are also useful techniques for revealing the localisation locus and cause of the pathological discrepancies.

Gy. Balka – M. Rusvai – S. Kecskeméti – I. Kiss:
PRRS – NEW CHALLENGE IN THE PORCINE SECTOR. 1. CHARACTERISTICS OF THE VIRUS. LITERATURE REVIEW AND OWN EXPERIENCES

Porcine respiratory and reproductive syndrome (PRRS) is a viral disease spread worldwide, and spreading also in Hungary, causing high economic losses, which, according to Act CLXXVI of 2005, is a notifiable disease in Hungary again since 1st January 2006. The authors in their articles in three parts summarise, on the basis of up-to-date data, the history of the disease, the most important characteristics of the causative agent, important elements of the epidemiology and pathogenesis of the disease. They present the diagnostic methods, the opportunities and challenge of control and the results of their own scientific studies in this subject. The first part presents the knowledge on PRRS-virus, emphasising the characteristic of the causative agent affecting the virulence, pathogenesis, diagnostics and control.

V. Halas – M. Kovács – L. Babinszky:
IMPACT OF NUTRIENT SUPPLY ON THE IMMUNE FUNCTIONS IN PIG. LITERATURE REVIEW

In recent years the nutritional research focused on the impact of different nutrients on the immunity of livestocks. Unlike numerous publications in this field, the present review discusses the effect of most important nutrients (protein, amino acids, fatty acids, carbohydrates) on the immune functions in pigs, considering the nutrient requirements.
The authors reviewed nearly 100 papers and drawn the following conclusions: The nutrients recommendations for pigs, as regards the requirements of maintenance and growth are not always sufficient for the adequate immune function. The results of different studies show that moderate protein deficiency does not reduce immune response. However, increased intake of some amino acids (methionine, threonine, arginine, glutamine or glutamic acid), compared to the nutrient requirement of maintenance and growth may result in better immunity. Therefore, among essential amino acids the recommended methionine, threonine, arginine supply will certainly be overviewed in the near future. In case of some nutrients (unsaturated fatty acids) the overdose may have immune supressive effect before the performance reduced. However, it has to be noted that literature data refer to studies, in which different dosages, irrespective to recommendation were used to study the animal response. It is possible that both the immunsuppress and the reduced performance were caused by the change in nutrient ratio, rather than only by the exaggerated nutrient supply. Despite the fact that the impact of nutrient supply on immune functions has to be more studied, it can be stated that the diet formulation by optimizing the diet components and nutrient intake can help to achieve better animal health and immunity.

P. Zenke − Zs. Pádár − L. Zöldág:
MOLECULAR GENETICS AND DOG BREEDING

Hidden within the genome of every single individual there is a characteristic and unique „DNA signature” recognizable and identifiable by experts being proficient in reading. Molecular techniques are usable for direct detection of specific segments – genetic markers/alleles – of the DNA. The alleles are inherited, usually 50% from each parent. Depending on the number and variance of the applied microsatellite loci, the identity and pedigree of an individual can be determined, even in highly inbred populations. Molecular genetics are used widely abroad in kennel clubs to determine the parentage of dogs. The DNA registry of purebred dogs also provides a solid foundation for research on genetic diseases, help on forecasting them and to filter out carriers from breeding. In forensic aspect it plays a significant role in clear up and proof serious crimes in addition to dog attacks. In Hungary the canine identification has initiated for forensic purposes since 2000, when 10 microsatellite loci were examined with a PCR multiplex kit. Population-statistical analysis using the observed allele frequency data of breeds and breed groups was performed, comparing e. g. heterozygosity, power of discrimination and exclusion, as well as the rates of inbreeding between purebred subpopulations. On the bases of the results, the authors call the attention to the advantages, topicality and actuality of the investigation and the further – possible and expected – wide-range application of it.

K. Lefler – J. Gál – Gy. Demeter:
EFFECT OF WATER TEMPERATURE ON THE EMBRYONIC DEVELOPMENT AND HATCHING RESULTS OF RAINBOW TROUT (ONCORHYNCHUS MYKISS)

The authors hatched rainbow trout eggs in water of different temperature but of the same oxygen content and reaction. They found that at 7.55 oC hatching started on 43rd day and terminated on 45th day. There were no developmental anomalies on the spawns hatched at this temperature. With the increase of water temperature hatching time decreased. High mortality and developmental anomalies were observed in case of hatching at 13.42 oC. From developmental anomalies vertebral column deformities, bicephaly, twin development defects and distortion of tail and tail fin occur. The authors call the attention with their studies to the negative effect of the increase of temperature of reproductive environment in case of species living in mountain and submountain areas, using rainbow trout as a model.