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Last edited 
by the webmaster:
20-09-2006

DEPARTMENT AND CLINIC OF OBSTETRICS AND REPRODUCTION

Research activity of the department

Basically divided into 3 sections:

• Prof. Solti and his team: Reproductive biotechnology including embryo transfer and related subjects (superovulation, splitting of embryos, cryopreservation, cloning by nuclear transfer, microinjection of early embryos etc.). Determination of hormones and some mycotoxins by ELISA, development of rapid on-farm immunoassays. Infertility of male animals; evaluation and treatment of infertile males in different species. Assisted reproductive technologies applied in different species. Computer assisted semen analysis.

• Prof. Szenci and his team: Embryonic mortality, reproductive ultrasonography in cattle. Pregnancy-associated proteins. Calcium-metabolism in the peripartal period and uterine involution in cattle, respiration physiology of the newborn calf.

• Prof. Huszenicza and his team: Effects of nutrition / feeding technology and energy metabolism on resumption of cyclic ovarian activity in the postpartum period and at the beginning of the breeding season (ruminants, horse). Endocrine and ovarian consequences of endotoxin/cytokine-mediated diseases (mastitis, metritis). Ovarian characteristics of pet ferrets (Mustela putorius furo).

Laboratory for Andrology and Assisted Reproduction (LAAR)

(Head: Prof. Dr. Sándor Cseh DVM, Ph.D., DSc, Dipl. ECAR)

The LAAR was established in 1999 and has 3 important fields of activities: education, services and research. The lab is well-equipped for performing a wide variety of experiments/investigations and laboratory tests on the field of assisted reproduction (e.g. cryopreservation of gametes /semen, egg and embryo/, in vitro fertilization, embryo transfer, artificial insemination, oestrus synchronization, ovulation induction, etc.) and andrology (e.g. traditional and computer assisted semen analyses /CASA/, evaluation of fertilizing capability /potential breeding soundness/ of males, infertility treatment of male animals, different sperm treatment protocols, etc.).

Section for Reproductive Pathology and Clinical Endocrinology

(Head: Prof. Dr. Gyula Huszenicza DVM, Ph.D., Dipl. ECAR)

This section was established in 1989. They offer wide range of validated ³H- and ¹²⁵I-RIA and ELISA techniques for assaying various hormones in blood (plasma or serum progesterone, 17-estradiol, eston sulphate, testosterone, cortisol, 3,3’,5-triiodothyronine, thyroxine, insulin, insulin-like growth factor-1, leptin and LH), milk (progesterone), feces (progesterone metabolites) and urine (cortisol). This unit is the professional organizer of an annual summer school-like PhD course system (Budapest Workshop for Young Endocrinologist), which is recognized as an official course of the European Society of Domestic Animal Reproduction (ESDAR), and the European College of Animal Reproduction (ECAR) nowadays.

Small Animal Clinic of Obstetrics and Reproduction

(Head: Dr. Julianna Thuróczy DVM, Ph.D., Dipl. ECAR)

The clinic provides a 24-hours service for small animal -primarily canine and feline- patients, which covers daily general ambulatory service (e.g. obstetrical ultrasonography, estrus detection and determination of optimal timing of mating, routine obstetrical surgery /spaying and castration/, vaginal plastic surgery /for vaginal prolapse and tumors/, removal of mammary tumors, artificial insemination, etc.) and non-stop emergency care (e.g. obstetrical aid for patients with dystocia, caesarean section, intensive care for pregnant bitches with metabolic disease). It also offers special consultation possibilities in different areas of canine and feline obstetrics such as pregnancy care, infertility evaluation, andrology and assisted reproduction, and neonatology.

Selected recent publications from the department:

Thuróczy, J., Rijnberk, A., van Sluijs, F.J., Kooistra, H.S., Voorhout, G., Mol, J.A., van der Linde-Sipman, J.S. (1998): Multiple endocrine neoplasia in a dog: Corticotrophic tumour, bilateral adrenocortical tumours and pheochromocytoma. Veterinary Quarterly: 20(1):1-7.

Cseh, S., Chan, PJ., Corselli, J., Bailey, L. (2000): Electroejaculated baboon (Papio anubis) sperm requires a higher dosage of pentoxifylline to enhance motility. Journal of Assisted Reproduction and Genetics, 17:449-453.

Huszenicza, Gy., Fekete, S., Szigeti, G., Kellems, R.O., Kulcsár, M., Fébel, H. Nagy, P., Cseh, S., Veresegyházy, T., Hullár, I. (2000): Ovarian consequences of low dose peroral Fusarium (T-2) toxin intake in ewe and heifer model. Theriogenology, 53:1631-1639.

Barna-Vetró, I., Szabó, E., Fazekas, B., Solti, L. (2000): Development of a sensitive ELISA for determination of Fumonisin B1 in cereals. J. Agricultural and Food Chemistry. 48(7): 2821-2825.

Solti, L., Crichton, E. G., Loskutoff, N. M., Cseh, S. (2000): Economical and ecological importance of indigenous livestock and the application of assised reproduction to their preservation. Theriogenology, 53:149-162.

Rátky, J., Brüssow, K.-P., Solti, L., Torner, H., Sarlós, P. (2001): Ovarian response, embryo recovery and results of embryo transfer in a Hungarian native pig breed. Theriogenology, 56: 969-978.

Szász, F., Sirivaidyapong, S., Cheng, F.P., Voorhout, W. F., Marks, A., Colenbrander, B., Solti, L., Gadella, B. M. (2000): Detection of calcium ionophore induced membrane changes in dog sperm as a simple method to predict the cryopreservability of dog semen. Journal of Molecular Reproduction and Development, 55:289-298.

Cseh, S., Corselli, J., Chan, P., Bailey, L. (2001): Controlled ovarian stimulation and ultrasound-guided follicular aspiration in baboon. Reproduction, Nutrition, Development, 41 (6):531-534.

Jánosi, Sz., Szigeti, G., Rátz, F., Laukó, T., Kerényi, J., Tenk, M., Katona, F., Huszenicza, A., Kulcsár, M., Huszenicza, Gy. (2001): Prototheca zopfii mastitis in dairy cows under continental climatic conditions. Veterinary Quarterly, 23. 80-83.

Balogh, L., Thuróczy, J., Andócs, G., Máthé, D., Chaudhari, P., Perge, E., Biksi, I., Polyák, A., Király, R., Jánoki, Gy A. (2002): Sentinel lymph node detection in canine oncological patients. Nuclear Medicine Review, 5(2):139-144.

Biró, K., Solti, L., Barna-Vetró, I., Bagó, Gy., Glávits, R., Szabó, E., Fink-Gremmels, J. (2002): Tissue distribution of ochratoxin A as determined by HPLC and ELISA and histopathological effects in chickens. Avian Pathology, 31:141-148.

Cseh, S., Corselli, J., Chan, P., Bailey, L. (2002): Superovulation using recombinant human FSH and ultrasound-guided transabdominal follicular aspiration in baboon (Papio anubis). Animal Reproduction Science, 70(3-4):287-293.

Taponen, J., Kulcsár, M., Kátai, L., Huszenicza, Gy., Rodríguez-Martínez, H., Katila, T. (2002): Short oestrous cycles and oestrous signs after premature ovulations induced with cloprostenol and gonadotropin-releasing hormone in cyclic dairy cows. Theriogenology, 58:1291-1302.

Biró, K., Barna-Vetró, I., Pécsi, T., Szabó, E., Winkler, G., Fink-Gremmels, J., Solti, L. (2003): Evaluation of spermatological parameters in ochratoxin A-challenged boars. Theriogenology, 60:199-207.

Nikolic, J. A., Kulcsár, M., Kátai, L., Nedic, O., Jánosi, Sz., Huszenicza, Gy. (2003): Periparturient endocrine and metabolic changes in healthy cows and in those affected by various forms of mastitis. Journal of Veterinary Medicine A, 50:22-29.

Thuróczy, J., Wölfling, A., Tibold, A., Balogh, L., Jánoki, Gy. A., Solti, L. (2003): Effect of anticoagulants and sampling time on results of progesterone determination in canine blood samples. Reproduction of Domestic Animals, 38:386-390.

Nagy P., Huszenicza Gy., Reiczigel J., Juhász J., Kulcsár M., Abaváry K., Guillaume D. (2004): Factors affecting plasma progesterone concentration and the retrospective determination of time of ovulation in cyclic mares. Theriogenology, 61. 203-214.